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Anti-TFF3 Polyclonal Antibody

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货号 TMAB-01824

Anti-TFF3 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TFF3。Anti-TFF3 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P。

Anti-TFF3 Polyclonal Antibody

Anti-TFF3 Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-01824

Anti-TFF3 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TFF3。Anti-TFF3 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍


生物活性
产品描述
Anti-TFF3 Polyclonal Antibody is a Rabbit antibody targeting TFF3. Anti-TFF3 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.
Ig Type
IgG
反应种属
Human,Mouse,Rat (predicted:Cow)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (TMAB-01824) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated (TMAB-01824) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (TMAB-01824) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (TMAB-01824) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (TMAB-01824) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (TMAB-01824) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用IFIHC-FrIHC-P
推荐剂量
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted, extracellular space, extracellular matrix, apical lamina.
组织特异性Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
抗原信息
免疫原
KLH conjugated synthetic peptide: human TFF3
抗原种属
Human
基因名称
TFF3
基因ID
蛋白名称
Trefoil factor 3
Uniprot ID
研究领域
Other ECM Proteins
化学信息
分子量Theoretical: 6.5 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

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