Anti-TBX1 Polyclonal Antibody 2 is a Rabbit antibody targeting TBX1. Anti-TBX1 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse)

1. Sample: Brain (Rat) Lysate at 40 μg
Primary: Anti-TBX1 (TMAB-13404) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 43 kD
Observed band size: 44 kD
2. Sample: Muscle (Mouse) Lysate at 40 μg
Primary: Anti-TBX1 (TMAB-13404) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
3. Blank control: K562.
Primary Antibody (green line): Rabbit Anti-TBX1 antibody (TMAB-13404)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
4. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Heart tissue lysates
Lane 3: Human K562 cell lysates
Lane 4: Human 293T cell lysates
Primary: Anti-TBX1 (TMAB-13404) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human TBX1

Human

TBX1

T-box transcription factor TBX1

Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries.
Subunit : Interacts with DSCR6.