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Anti-Syndecan-1 Polyclonal Antibody

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货号 TMAB-01804

别名 Syndecan-1, syndecan 1, syndecan, SYND1, SDC, CD138

Anti-Syndecan-1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Syndecan-1。Anti-Syndecan-1 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

Anti-Syndecan-1 Polyclonal Antibody

Anti-Syndecan-1 Polyclonal Antibody

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货号 TMAB-01804 别名 Syndecan-1, syndecan 1, syndecan, SYND1, SDC, CD138

Anti-Syndecan-1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Syndecan-1。Anti-Syndecan-1 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

规格价格库存数量
50 μL
¥ 1,170
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100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍


生物活性
产品描述
Anti-Syndecan-1 Polyclonal Antibody is a Rabbit antibody targeting Syndecan-1. Anti-Syndecan-1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
别名Syndecan-1, syndecan 1, syndecan, SYND1, SDC, CD138
Ig Type
IgG
反应种属
Human,Mouse,Rat,Cow (predicted:Chicken,Dog,Pig,Horse,Rabbit)
验证活性
1. Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Human U251 cell lysates
Lane 4: Human A431 cell lysates
Lane 5: Human HepG2 cell lysates
Lane 6: Human HeLa cell lysates
Primary: Anti-Syndecan-1 (TMAB-01804) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 74 kDa
2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (TMAB-01804) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (TMAB-01804) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (TMAB-01804) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
verifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用IFIHC-FrIHC-PWB
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Membrane; Single-pass type I membrane protein. Secreted. Note=Shedding of the ectodomain produces a soluble form.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]
抗原信息
免疫原
KLH conjugated synthetic peptide: human CD138
抗原种属
Human
基因名称
SDC1
基因ID
蛋白名称
Syndecan-1
Uniprot ID
研究领域
Metabolism of lipids and lipoproteins,Extracellular matrix,Lipid metabolism,Cancer,ECM Proteins,Membrane Proteins,Synapse marker,Neurogenesis,Glucosaminoglycans,HSPGs,B Lymphocytic Lineage
功能
Cell surface proteoglycan that bears both heparin sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
化学信息
分子量Theoretical: 34 kDa. Actual: 60-70 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

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