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Anti-Syndecan-1 Polyclonal Antibody
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产品编号 TMAB-01804
Anti-Syndecan-1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Syndecan-1。Anti-Syndecan-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Syndecan-1 Polyclonal Antibody
Anti-Syndecan-1 Polyclonal Antibody
还可以产品编号 TMAB-01804
Anti-Syndecan-1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Syndecan-1。Anti-Syndecan-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Syndecan-1 Polyclonal Antibody is a Rabbit antibody targeting Syndecan-1. Anti-Syndecan-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat,Cow (predicted:Chicken,Dog,Pig,Horse,Rabbit) |
| 验证活性 | 1. Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Liver tissue lysates Lane 3: Human U251 cell lysates Lane 4: Human A431 cell lysates Lane 5: Human HepG2 cell lysates Lane 6: Human HeLa cell lysates Primary: Anti-Syndecan-1 (TMAB-01804) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 74 kDa 2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (TMAB-01804) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (TMAB-01804) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (TMAB-01804) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. Secreted. Note=Shedding of the ectodomain produces a soluble form. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008] |
| 免疫原 | KLH conjugated synthetic peptide: human CD138 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Metabolism of lipids and lipoproteins,Extracellular matrix,Lipid metabolism,Cancer,ECM Proteins,Membrane Proteins,Synapse marker,Neurogenesis,Glucosaminoglycans,HSPGs,B Lymphocytic Lineage |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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