Anti-SYK Polyclonal Antibody is a Rabbit antibody targeting SYK. Anti-SYK Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow)

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-SYK Polyclonal Antibody, Unconjugated (TMAB-01800) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-SYK (TMAB-01800) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 72/69 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human SYK

Human

Platelets,Mast Cells,Tyrosine Kinases