Anti-Stra8 Polyclonal Antibody is a Rabbit antibody targeting Stra8. Anti-Stra8 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Mouse,Rat (predicted:Human)

1. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Stra8) Polyclonal Antibody, Unconjugated (TMAB-01793) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Testis (Mouse) Lysate at 40 μg
Primary: Anti-TMAB-01793 (TMAB-01793) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
3. Sample:
Testis (Rat) Lysate at 40 μg
Primary: Anti-TMAB-01793 (TMAB-01793) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Stra8

Human

STRA8

Stimulated by retinoic acid gene 8 protein homolog

Surface molecules,Germ cell markers,Intracellular,Embryonic Germ Cells

Required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase