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Anti-STK31 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 STK31。Anti-STK31 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-STK31 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 STK31。Anti-STK31 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
| 产品描述 | Anti-STK31 Polyclonal Antibody is a Rabbit antibody targeting STK31. Anti-STK31 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 反应种属 | Human,Mouse,Rat |
| 验证活性 | 1. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-STK31 Polyclonal Antibody, Unconjugated (TMAB-13226) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Sample: MCF-7 (Human) Cell Lysate at 30 μg A431 (Human) Cell Lysate at 30 μg Primary: Anti-STK31 (TMAB-13226) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 116 kD Observed band size: 116 kD 3. Sample: Testis (Mouse) Lysate at 40 μg A431 (Human) Cell Lysate at 30 μg Primary: Anti-STK31 (TMAB-13226) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 116 kD Observed band size: 116 kD |
| 应用 | WBIHC-PIHC-FrIF |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 组织特异性 | Testis specific. |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | STK31 (Serine/threonine protein kinase 31) is similar to a mouse gene that encodes a putative protein kinase with a tudor domain, and shows testis specific expression. Alternative splicing results in multiple transcript variants encoding different isoforms. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. |
| 免疫原 | KLH conjugated synthetic peptide: human STK31 |
| 抗原种属 | Human |
| 基因名称 | STK31 |
| 基因ID | |
| 蛋白名称 | Serine/threonine-protein kinase 31 |
| Uniprot ID |
| 分子量 | Theoretical: 116 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |