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Anti-STAT1 Antibody (5F189)
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产品编号 TMAC-03864
别名 STAT91, signal transducer and activator of transcription 1, 91kDa, ISGF-3, IMD31C, IMD31B, IMD31A, CANDF7
Anti-STAT1 Antibody (5F189) 是一种 Rabbit 抗体，靶向 STAT1。Anti-STAT1 Antibody (5F189) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-STAT1 Antibody (5F189)
Anti-STAT1 Antibody (5F189)
还可以产品编号 TMAC-03864 别名 STAT91, signal transducer and activator of transcription 1, 91kDa, ISGF-3, IMD31C, IMD31B, IMD31A, CANDF7
Anti-STAT1 Antibody (5F189) 是一种 Rabbit 抗体，靶向 STAT1。Anti-STAT1 Antibody (5F189) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-STAT1 Antibody (5F189) is a Rabbit antibody targeting STAT1. Anti-STAT1 Antibody (5F189) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | STAT91, signal transducer and activator of transcription 1, 91kDa, ISGF-3, IMD31C, IMD31B, IMD31A, CANDF7 |
| Ig Type | IgG |
| 克隆号 | 5F189 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of STAT1 on different lysates using anti-STAT1 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293. 2. Immunohistochemical analysis of paraffin-embedded human cervix uteri tissue using anti-STAT1 alpha antibody. Counter stained with hematoxylin. 3. ICC staining STAT1 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining STAT1 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining STAT1 alpha in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of MCF-7 cells with STAT1 alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 87 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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