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Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody
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产品编号 TMAB-01766
Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Sphingosine Kinase 1/SPHK1。Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody
Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody
还可以产品编号 TMAB-01766
Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Sphingosine Kinase 1/SPHK1。Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody is a Rabbit antibody targeting Sphingosine Kinase 1/SPHK1. Anti-Sphingosine Kinase 1/SPHK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Mouse,Rat (predicted:Human,Dog,Cow,Rabbit) |
| 验证活性 | 1. Sample: Heart (Mouse) Lysate at 30 μg Primary: Anti-SPHK1 (TMAB-01766) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SPHK1) Polyclonal Antibody, Unconjugated (TMAB-01766) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Lane 1: Mouse Pancreas tissue lysates Lane 2: Mouse Lung tissue lysates Lane 3: Rat Kidney tissue lysates Primary: Anti-SPHK1 (TMAB-01766) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42-52 kDa Observed band size: 50 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| 免疫原 | KLH conjugated synthetic peptide: human SPHK1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Metabolism of lipids and lipoproteins,Lipid metabolism,GPCR,Lipid Kinases |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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