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Anti-SPARCL1 Polyclonal Antibody
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产品编号 TMAB-01765
Anti-SPARCL1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 SPARCL1。Anti-SPARCL1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-SPARCL1 Polyclonal Antibody
Anti-SPARCL1 Polyclonal Antibody
还可以产品编号 TMAB-01765
Anti-SPARCL1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 SPARCL1。Anti-SPARCL1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-SPARCL1 Polyclonal Antibody is a Rabbit antibody targeting SPARCL1. Anti-SPARCL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Mouse,Rat (predicted:Human,Pig,Horse,Rabbit) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (SPARCL1) Polyclonal Antibody, Unconjugated (TMAB-01765) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 2. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-SPARCL1 (TMAB-01765) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 73 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Secreted, extracellular space, extracellular matrix. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | SPARC (secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC-like protein 1 (SPARCL1), also known as high endothelial venule protein (Hevin) or MAST9, is a 664 amino acid member of the SPARC family of proteins. Highly expressed in lymph node, heart, lung, brain, skeletal muscle, ovary, colon and small intestine, SPARCL1 is a secreted protein that contains one EF-hand domain, one follistatin-like domain and one Kazal-like domain. SPARCL1 is implicated to play a role in neuronal remodeling and tumor suppression. The gene encoding SPARCL1 maps to chromosome 4q22.1. |
| 免疫原 | KLH conjugated synthetic peptide: human SPARCL1/Ecm2 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Tumor suppressors,Tumor biomarkers |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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