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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-SPARCL1 Polyclonal Antibody

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货号 TMAB-01765

别名 SPARC like 1, Sc1, mast9, hevin, Ecm2

Anti-SPARCL1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 SPARCL1。Anti-SPARCL1 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

Anti-SPARCL1 Polyclonal Antibody

Anti-SPARCL1 Polyclonal Antibody

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货号 TMAB-01765 别名 SPARC like 1, Sc1, mast9, hevin, Ecm2

Anti-SPARCL1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 SPARCL1。Anti-SPARCL1 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-SPARCL1 Polyclonal Antibody is a Rabbit antibody targeting SPARCL1. Anti-SPARCL1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
别名SPARC like 1, Sc1, mast9, hevin, Ecm2
Ig Type
IgG
反应种属
Mouse,Rat (predicted:Human,Pig,Horse,Rabbit)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (SPARCL1) Polyclonal Antibody, Unconjugated (TMAB-01765) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-SPARCL1 (TMAB-01765) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 73 kDa
verifiedActivityverifiedActivity
应用IFIHC-FrIHC-PWB
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted, extracellular space, extracellular matrix.
组织特异性Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景SPARC (secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC-like protein 1 (SPARCL1), also known as high endothelial venule protein (Hevin) or MAST9, is a 664 amino acid member of the SPARC family of proteins. Highly expressed in lymph node, heart, lung, brain, skeletal muscle, ovary, colon and small intestine, SPARCL1 is a secreted protein that contains one EF-hand domain, one follistatin-like domain and one Kazal-like domain. SPARCL1 is implicated to play a role in neuronal remodeling and tumor suppression. The gene encoding SPARCL1 maps to chromosome 4q22.1.
抗原信息
免疫原
KLH conjugated synthetic peptide: human SPARCL1/Ecm2
抗原种属
Human
基因名称
SPARCL1
基因ID
8404
蛋白名称
SPARC-like protein 1
Uniprot ID
Q14515
研究领域
Tumor suppressors,Tumor biomarkers
化学信息
分子量Theoretical: 74 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

计算器

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