购物车
- 全部删除
您的购物车当前为空
Anti-SPARC Antibody (8C653)
还可以产品编号 TMAY-03324
Anti-SPARC Antibody (8C653) 是一种 Rabbit 抗体，靶向 SPARC。Anti-SPARC Antibody (8C653) 可用于 WB,ELISA,IP。
Anti-SPARC Antibody (8C653)
Anti-SPARC Antibody (8C653)
还可以产品编号 TMAY-03324
Anti-SPARC Antibody (8C653) 是一种 Rabbit 抗体，靶向 SPARC。Anti-SPARC Antibody (8C653) 可用于 WB,ELISA,IP。
产品信息正在更新中，如果您想购买，请点击大包装&定制按钮.
大包装 & 定制
联系我们获取更多批次信息
资源下载
产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-SPARC Antibody (8C653) is a Rabbit antibody targeting SPARC. Anti-SPARC Antibody (8C653) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 8C653 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Anti-SPARC rabbit monoclonal antibody at 1:500 dilution. -Lane A: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:35 kDa. -Observed band size:40 kDa. 2. Mouse SPARC was immunoprecipitated using: -Lane A:0.5 mg A549 Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate -0.5 µL anti-Mouse SPARC rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse SPARC rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 35 kDa. -Observed band size: 38 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Osteonectin / SPARC (rM Osteonectin / SPARC; TMPY-01796; NP_033268.1; Met1-Ile302). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Secreted protein acidic and rich in cysteine (SPARC), also known as Osteonectin (ON), is a member of the SPARC family. SPARC consists of three domains: an EF-hand domain, a follistatin-like domain and a Kazal-like domain, and each of which has independent activity and unique properties. The activity of SPARC is context- and cell-type-dependent, which is highlighted by the fact that SPARC has shown seemingly contradictory effects on tumor progression in both clinical correlative studies and in animal models. The location of SPARC in the nuclear matrix of certain proliferating cells, but only in the cytosol of postmitotic neurons, indicates potential functions of SPARC as a nuclear protein, which might be involved in the regulation of cell cycle progression and mitosis. It functions not only to modulate cell-cell and cell-matrix interactions, but its de-adhesive and growth inhibitory properties in non-transformed cells have led to studies to assess its role in cancer. Its divergent actions reflect the complexity of this protein, because in certain types of cancers, such as melanomas and gliomas, SPARC is associated with a highly aggressive tumor phenotype, while in others, mainly ovarian, neuroblastomas and colorectal cancers, SPARC may function as a tumor suppressor. Recent studies have also demonstrated a role for SPARC in sensitizing therapy-resistant cancers. Notably, SPARC is linked to human obesity. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Mouse Osteonectin / SPARC protein (TMPY-01796) |
| 抗原种属 | Mouse |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
评论列表
预览
Related Tags: buy Anti-SPARC Antibody (8C653) | purchase Anti-SPARC Antibody (8C653) | Anti-SPARC Antibody (8C653) cost | order Anti-SPARC Antibody (8C653)
嗨！有任何问题？点我咨询
评论内容