Anti-SOX10 Antibody (1N16) is a Rabbit antibody targeting SOX10. Anti-SOX10 Antibody (1N16) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

1N16

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SOX10 Monoclonal Antibody, Unconjugated (TMAB-13054) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SOX10 Monoclonal Antibody, Unconjugated (TMAB-13054) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. 25 μg total protein per lane of various lysates (see on figure) probed with SOX10 monoclonal antibody, unconjugated (TMAB-13054) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
4. 4% Paraformaldehyde-fixed A375 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (SOX10) monoclonal Antibody, unconjugated (TMAB-13054) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200

Monoclonal

SOX10

Transcription factor SOX-10

Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.