Anti-Sodium hydrogen exchanger 1 Polyclonal Antibody is a Rabbit antibody targeting Sodium hydrogen exchanger 1. Anti-Sodium hydrogen exchanger 1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Sample:
Brain (Rat) lysate at 30 μg;
Liver (Rat) lysate at 30 μg;
Primary: Anti-NHE1 (TMAB-01752) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (bse-0295G) at 1: 3000 dilution;
Predicted band size: 91 kDa
Observed band size: 91 kDa
We are unsure as to the identity of these extra bands
2. Sample:
molt-4 (human) cell Lysate at 40 μg
Primary: Anti-Sodium hydrogen exchanger 1 (TMAB-01752) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kDa
Observed band size: 91 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated (TMAB-01752) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NHE1

Human

SLC9A1

Sodium/hydrogen exchanger 1

Sodium/ion transport,Cancer,Heart disease,Transmembrane,Channels

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.