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Anti-SOD1 Antibody (8C907)
还可以产品编号 TMAY-02022
别名 superoxide dismutase 1, soluble, SOD, IPOA, hSod1, homodimer, HEL-S-44, ALS1, ALS
Anti-SOD1 Antibody (8C907) 是一种 Mouse 抗体，靶向 SOD1。Anti-SOD1 Antibody (8C907) 可用于 WB,FCM,IP。
Anti-SOD1 Antibody (8C907)
Anti-SOD1 Antibody (8C907)
还可以产品编号 TMAY-02022 别名 superoxide dismutase 1, soluble, SOD, IPOA, hSod1, homodimer, HEL-S-44, ALS1, ALS
Anti-SOD1 Antibody (8C907) 是一种 Mouse 抗体，靶向 SOD1。Anti-SOD1 Antibody (8C907) 可用于 WB,FCM,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 1,694 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-SOD1 Antibody (8C907) is a Mouse antibody targeting SOD1. Anti-SOD1 Antibody (8C907) can be used in WB,FCM,IP. |
| 别名 | superoxide dismutase 1, soluble, SOD, IPOA, hSod1, homodimer, HEL-S-44, ALS1, ALS |
| Ig Type | Monoclonal Mouse IgG2b |
| 克隆号 | 8C907 |
| 交叉反应 | Human |
| 验证活性 | 1. Flow cytometric analysis of Human SOD1 expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-SOD1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Anti-SOD1 mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: Hela Whole Cell Lysate. -Lane C: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:16 kDa. -Observed band size:20 kDa. 3. SOD1 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg HepG2 Whole Cell Lysate -0.5 µL anti-SOD1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-SOD1 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 16 kDa. -Observed band size: 18 kDa |
| 应用 | WB,FCM,IP |
| 推荐剂量 | WB: 1:500-1:1000; FCM: 1:100-1:500; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SOD1 / Superoxide Dismutase (rh SOD1 / Superoxide Dismutase; TMPY-01585; NP_000445.1; Ala 2-Gln 154). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | SOD1 belongs to the Cu-Zn superoxide dismutase family. It binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. SOD1 destroys radicals which are normally produced within the cells and which are toxic to biological systems. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1). ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | FITC/PE |
| 可提供抗体种类 | 3 |
| 免疫原 | Recombinant Human SOD1 / Superoxide Dismutase protein (TMPY-01585) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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