Anti-SOD1 Antibody (7K225) is an antibody targeting SOD1. Anti-SOD1 Antibody (7K225) can be used in ELISA, WB, FC.

Rabbit IgG

7K225

Human

1. Western Blot
-Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate
-All lanes: SOD1 antibody at 1:1500
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 16 kDa
-Observed band size: 18 kDa
2. Overlay histogram showing Jurkat cells stained with TMAH-01120 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, FC

WB:1:500-1:5000; FC:1:20-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human SOD1

Human

6647

Cancer, Cell biology, Metabolism, Signal transduction