Anti-SMAD1/SMAD5 Polyclonal Antibody is a Rabbit antibody targeting SMAD1/SMAD5. Anti-SMAD1/SMAD5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rabbit (predicted:Rat,Chicken,Dog,Cow,Horse)

1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (SMAD1 + SMAD5) polyclonal Antibody, Unconjugated (TMAB-01735) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SMAD1 + SMAD5) Polyclonal Antibody, Unconjugated (TMAB-01735) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-SMAD1 + SMAD5 (TMAB-01735) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 55 kDa

WB: 1:200-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human SMAD5

Human

Liver development,SMADs,SMADs,Cytoplasmic