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顶部生物活性储存和溶解信息计算器

Anti-SLC32A1 Polyclonal Antibody

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Anti-SLC32A1 Polyclonal Antibody
产品编号 TMAB-01719

Anti-SLC32A1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 SLC32A1。Anti-SLC32A1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-SLC32A1 Polyclonal Antibody

Anti-SLC32A1 Polyclonal Antibody

Rating icon 还可以
Anti-SLC32A1 Polyclonal Antibody
产品编号 TMAB-01719

Anti-SLC32A1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 SLC32A1。Anti-SLC32A1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,165
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100 μL
¥ 1,965
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200 μL
¥ 2,790
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产品介绍

生物活性
产品描述
Anti-SLC32A1 Polyclonal Antibody is a Rabbit antibody targeting SLC32A1. Anti-SLC32A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Mouse,Rat (predicted:Human,Pig,Cow,Rabbit)
验证活性
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-SLC32A1 Polyclonal Antibody, Unconjugated (TMAB-01719) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: Cerebellum (Mouse) Lysate at 40 μg
Primary: Anti-SLC32A1 (TMAB-01719) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
3. Sample: Heart (Mouse) Lysate at 40 μg
Primary: Anti-SLC32A1 (TMAB-01719) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa
应用
WB
IHC-P
IHC-Fr
IF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasmic vesicle membrane; Multi-pass membrane protein
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景Synaptic transmission involves the controlled exocytosis of vesicles containing specific neurotransmitters. Usually, neurotransmitters are synthesized in the cytoplasm of the cell and must be transported into synaptic vesicles for release. The vesicular GABA transporter (VGAT) is responsible for loading γ-aminobutyric acid (GABA), an inhibitory neurotransmitter, from neuronal cytoplasm into synaptic vesicles and is expressed only in the nerve endings of inhibitory neurons that contain GABA and/or glycine. During neocortical development, VGAT expression barely precedes the maturation of inhibitory synaptogenesis, suggesting that it may contribute to the development of neocortical GABAergic circuitry. VGAT may also play a role in epileptogenesis and the recovery mechanisms that occur after a spontaneous seizure.
抗原信息
免疫原
KLH conjugated synthetic peptide: human SLC30A9
抗原种属
Human
基因ID
140679
Uniprot ID
Q9H598
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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