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Anti-SLC2A6 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 SLC2A6。Anti-SLC2A6 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-SLC2A6 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 SLC2A6。Anti-SLC2A6 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,175 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-SLC2A6 Polyclonal Antibody is a Rabbit antibody targeting SLC2A6. Anti-SLC2A6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Mouse,Rat (predicted:Human) |
验证活性 | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-GLUT6 Polyclonal Antibody, Unconjugated (TMAB-01717) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-GLUT6 Polyclonal Antibody, Unconjugated (TMAB-01717) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Brain (Mouse) Lysate at 40 μg Primary: Anti-GLUT6/GLUT9 (TMAB-01717) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa 4. Sample: Heart (Mouse) Lysate at 40 μg Primary: Anti-GLUT6/GLUT9 (TMAB-01717) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cell membrane. The dileucine internalization motif is critical for intracellular sequestration. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The oxidation of glucose functions as the dominant source of metabolic energy for mammals. The plasma membrane is impermeable to glucose, so the cellular uptake of this important nutrient is achieved by facultative hexose transproters (Gluts). Gluts are integral membrane proteins that transport glucose and related hexoses. Glucose binds to a Glut on one side of the membrane which provokes a conformational change causing it to release glucose to the other side. Members of the Glut family may enhance the metabolic activity of tumor cells. Glut6 is part of the third out of three classes of Gluts. Glut6 is mainly expressed in the brain, spleen and peripheral leukocytes. It appears to be regulated by subcellular redistribution, because it is targeted to intracellular compartments by di-leucine motifs, recycling itself in a Dynamin-dependent manner. |
免疫原 | KLH conjugated synthetic peptide: human GLUT6 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Cancer,Channels |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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