Anti-SLC2A3 Antibody (8D585) is an antibody targeting SLC2A3. Anti-SLC2A3 Antibody (8D585) can be used in ELISA, WB, IHC, FC.

Rabbit IgG

8D585

Human

1. Western Blot
-Positive WB detected in: 293 whole cell lysate (20µg), JK whole cell lysate (20µg), Hela whole cell lysate (20µg), HepG2 whole cell lysate (20µg), SY5Y whole cell lysate (20µg)
-All lanes: SLC2A3 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 54 kDa
-Observed band size: 54 kDa
-Exposure time: 60s
2. IHC image of TMAH-01104 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-01104 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
4. IHC image of TMAH-01104 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
5. Overlay Peak curve showing MCF7 cells stained with TMAH-01104 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC, FC

WB:1:500-1:2000; IHC:1:50-1:200; FC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human SLC2A3

Human

6515

Cancer, Cell biology, Metabolism, Signal transduction