Anti-SLC25A11 Polyclonal Antibody is a Rabbit antibody targeting SLC25A11. Anti-SLC25A11 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Cow,Rabbit,Sheep,GuineaPig)

1. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-SLC25A15 (TMAB-12863) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
2. Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-SLC25A15 antibody (TMAB-12863)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Sample:
Lane 1: Human U251 cell lysates
Lane 2: Human A549 cell lysates
Primary: Anti-SLC25A11 (TMAB-12863) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 32 kDa

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human SLC25A15

Human

SLC25A11

Mitochondrial 2-oxoglutarate/malate carrier protein

Catalyzes the transport of 2-oxoglutarate across the inner mitochondrial membrane in an electroneutral exchange for malate or other dicarboxylic acids, and plays an important role in several metabolic processes, including the malate-aspartate shuttle, the oxoglutarate/isocitrate shuttle, in gluconeogenesis from lactate, and in nitrogen metabolism.