Anti-SLC22A17 Polyclonal Antibody 2 is a Rabbit antibody targeting SLC22A17. Anti-SLC22A17 Polyclonal Antibody 2 can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Sample:
Lane 1: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 2: U2os (Human) Cell Lysate at 30 μg
Lane 3: U87MG (Human) Cell Lysate at 30 μg
Lane 4: A549 (Human) Cell Lysate at 30 μg
Lane 5: Siha (Human) Cell Lysate at 30 μg
Primary:
Anti-SLC22A17 (TMAB-01707) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (TMAB-01707) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (TMAB-01707) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (TMAB-01707) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human SLC22A17

Human

SLC22A17

Solute carrier family 22 member 17

Channels

Cell surface receptor for LCN2 (24p3) that plays a key role in iron homeostasis and transport. Able to bind iron-bound LCN2 (holo-24p3), followed by internalization of holo-24p3 and release of iron, thereby increasing intracellular iron concentration and leading to inhibition of apoptosis. Also binds iron-free LCN2 (apo-24p3), followed by internalization of apo-24p3 and its association with an intracellular siderophore, leading to iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration and resulting in apoptosis (By similarity).