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别名 VGluT2, Vglut2, Vesicular glutamate transporter 2, Solute carrier family 17 member 6, Slc17a6, Dnpi, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Differentiation-associated BNPI
Anti-SLC17A6 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 SLC17A6。Anti-SLC17A6 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P。
Anti-SLC17A6 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 SLC17A6。Anti-SLC17A6 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
| 产品描述 | Anti-SLC17A6 Polyclonal Antibody is a Rabbit antibody targeting SLC17A6. Anti-SLC17A6 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P. |
| 别名 | VGluT2, Vglut2, Vesicular glutamate transporter 2, Solute carrier family 17 member 6, Slc17a6, Dnpi, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Differentiation-associated BNPI |
| Ig Type | IgG |
| 反应种属 | Rat (predicted:Human,Mouse,Chicken,Dog,Pig,Cow,Horse) |
| 验证活性 | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-VGLUT2 Polyclonal Antibody, Unconjugated (TMAB-01704) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Positive sample: Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Negative sample: Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VGLUT2) Polyclonal Antibody, Unconjugated (TMAB-01704) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. ![]() ![]() |
| 应用 | IFIHC-FrIHC-P |
| 推荐剂量 | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein (Potential). Cell junction, synapse, synaptosome. |
| 组织特异性 | Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus. |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1 mg/mL |
| 研究背景 | The ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi) cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic a and b cells express BNPI and VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI. |
| 免疫原 | KLH conjugated synthetic peptide: human VGLUT2 |
| 抗原种属 | Human |
| 基因名称 | SLC17A6 |
| 基因ID | |
| 蛋白名称 | Vesicular glutamate transporter 2 |
| Uniprot ID | |
| 研究领域 | Transporters |
| 功能 | Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. |
| 分子量 | Theoretical: 64 kDa. |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
| 存储 | store at low temperature | store at -20°C |