Anti-SHBG Polyclonal Antibody is a Rabbit antibody targeting SHBG. Anti-SHBG Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Cow,Rabbit)

1. Sample:
Lane1: Testis (Mouse) Lysate at 30 μg
Lane2: Liver (Mouse) Lysate at 30 μg
Primary: Anti-ABP/SHBg (TMAB-01683) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000 dilution;
Predicted band size: 40 kDa
Observed band size: 44 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (TMAB-01683) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (TMAB-01683) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (TMAB-01683) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
5. Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human U87MG cell lysates
Primary: Anti-SHBG (TMAB-01683) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 50 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human SHBG

Human

Cancer,Hormones,Lipid metabolism