Anti-SFTPA1 Polyclonal Antibody is a Rabbit antibody targeting SFTPA1. Anti-SFTPA1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.

IgG

Human (predicted:Mouse,Rat)

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-SFTPA1 Polyclonal Antibody, Unconjugated (TMAB-01681) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-SFTPA1 Polyclonal Antibody, Unconjugated (TMAB-01681) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Formalin-fixed and paraffin embedded mouse lung tissue labeled Anti-SFTPA1 Polyclonal Antibody, Unconjugated (TMAB-01681) at 1:2000, followed by conjugation to the secondary antibody and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human SFTPA2/SFTPA1

Human

SFTPA1

Pulmonary surfactant-associated protein A1

Other ECM Proteins

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.