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Anti-SERPINB1 Antibody (7K553) 是一种 Mouse 抗体,靶向 SERPINB1。Anti-SERPINB1 Antibody (7K553) 可用于 WB,IP。
Anti-SERPINB1 Antibody (7K553) 是一种 Mouse 抗体,靶向 SERPINB1。Anti-SERPINB1 Antibody (7K553) 可用于 WB,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μL | ¥ 1,694 | 5日内发货 |
产品描述 | Anti-SERPINB1 Antibody (7K553) is a Mouse antibody targeting SERPINB1. Anti-SERPINB1 Antibody (7K553) can be used in WB,IP. |
Ig Type | Monoclonal Mouse IgG1 |
克隆号 | 7K553 |
交叉反应 | Human |
验证活性 | 1. Anti-SERPINB1 mouse monoclonal antibody at 1:500 dilution. -Lane A: NCI-H460 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:43 kDa. -Observed band size:43 kDa. 2. SERPINB1 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate -0.5 µL anti-SERPINB1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-SERPINB1 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 43 kDa. -Observed band size: 43 kDa |
应用 | WB,IP |
推荐剂量 | WB: 1:500-1:1000; IP: 1-2 μL/mg of lysate |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SerpinB1 / ELANH2 (rh SerpinB1 / ELANH2; TMPY-02128; P30740; Met1-Pro379). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | SerpinB1 is an endogenous inhibitor of serine proteases recognized for its anti-inflammatory and host-protective properties. Serum SerpinB1 levels are elevated in patients with type 2 diabetes compared with that in healthy subjects and are negatively correlated with serum LDL-C. serpinB1 as an endogenous protein that can potentially be harnessed to enhance functional beta cell mass in patients with diabetes. |
偶联 | Unconjugated |
免疫原 | Recombinant Human SerpinB1 / ELANH2 protein (TMPY-02128) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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