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Anti-Semaphorin 3F Polyclonal Antibody 2

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货号 TMAB-12648

Anti-Semaphorin 3F Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 Semaphorin 3F。Anti-Semaphorin 3F Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-Semaphorin 3F Polyclonal Antibody 2

Anti-Semaphorin 3F Polyclonal Antibody 2

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Rating icon 还可以
货号 TMAB-12648

Anti-Semaphorin 3F Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 Semaphorin 3F。Anti-Semaphorin 3F Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,170
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100 μL
¥ 1,960
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-Semaphorin 3F Polyclonal Antibody 2 is a Rabbit antibody targeting Semaphorin 3F. Anti-Semaphorin 3F Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
反应种属
Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Rabbit,Sheep)
验证活性
1. Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Rat Brain Lysates
Primary: Anti-Semaphorin 3F (TMAB-12648) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 100 kDa
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Semaphorin 3F) Polyclonal Antibody, Unconjugated (TMAB-12648) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted.
组织特异性Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues(Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.
抗原信息
免疫原
KLH conjugated synthetic peptide: human Semaphorin 3F
抗原种属
Human
基因名称
SEMA3F
基因ID
6405
蛋白名称
Semaphorin-3F
Uniprot ID
Q13275
功能
May play a role in cell motility and cell adhesion.
化学信息
分子量Theoretical: 88 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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