Anti-SEMA4A Polyclonal Antibody is a Rabbit antibody targeting SEMA4A. Anti-SEMA4A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SEMA4A) Polyclonal Antibody, Unconjugated (TMAB-01674) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Kidney (Mouse) Lysate at 40 μg
Primary: Anti-SEMA4A (TMAB-01674) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 83 kDa
3. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-SEMA4A (TMAB-01674) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 83 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human SEMA4A

Human

Inhibitors,Axonal Guidance Proteins,Visual system