购物车
- 全部删除
您的购物车当前为空
Anti-SARS-CoV-2 Spike Antibody (3L24)
还可以产品编号 TMAY-02979
Anti-SARS-CoV-2 Spike Antibody (3L24) 是一种 Mouse 抗体，靶向 SARS-CoV-2 Spike。Anti-SARS-CoV-2 Spike Antibody (3L24) 可用于 WB,ELISA,IHC-P。
Anti-SARS-CoV-2 Spike Antibody (3L24)
Anti-SARS-CoV-2 Spike Antibody (3L24)
还可以产品编号 TMAY-02979
Anti-SARS-CoV-2 Spike Antibody (3L24) 是一种 Mouse 抗体，靶向 SARS-CoV-2 Spike。Anti-SARS-CoV-2 Spike Antibody (3L24) 可用于 WB,ELISA,IHC-P。
产品信息正在更新中，如果您想购买，请点击大包装&定制按钮.
大包装 & 定制
联系我们获取更多批次信息
资源下载
产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-SARS-CoV-2 Spike Antibody (3L24) is a Mouse antibody targeting SARS-CoV-2 Spike. Anti-SARS-CoV-2 Spike Antibody (3L24) can be used in WB,ELISA,IHC-P. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 3L24 |
| 验证活性 | 1. Anti-SARS-S1 mouse monoclonal antibody at 1:1000 dilution. -Lane A: SARS-CoV-2 (2019-nCoV) Spike S1 (30ng) -Lane B: SARS-CoV-2 (2019-nCoV) Spike S1 (5ng) -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. 2. Anti-SARS-CoV-2 (2019-nCoV) Spike mouse monoclonal antibody at 1:2000 dilution. -Lane A: SARS-COV-2 Spike (YP_009724390.1, WT) overexpressed HEK293 Whole Cell Lysate. -Lane B: HEK293 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. (Validation Experiment) 3. Immunochemical analysis of SARS-COV-2 Spike overexpressed HEK293 Cells were stained with purified anti-SARS-CoV-2 Spike Mouse Mab,then a HRP-conjugated second step antibody. (Validation Experiment) |
| 应用 | WB,ELISA,IHC-P |
| 推荐剂量 | WB: 1:2000-1:5000; ELISA: 1:1000-1:2000; IHC-P: 1:500-1:2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, Recombinant SARS-CoV-2 (2019-nCoV) Spike S1-mFc Protein (TMPY-05662; YP_009724390.1; Val16-Arg685). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant SARS-CoV-2 (2019-nCoV) Spike S1-mFc Protein (TMPY-05662) |
| 抗原种属 | SARS-CoV-2 |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
评论列表
预览
Related Tags: buy Anti-SARS-CoV-2 Spike Antibody (3L24) | purchase Anti-SARS-CoV-2 Spike Antibody (3L24) | Anti-SARS-CoV-2 Spike Antibody (3L24) cost | order Anti-SARS-CoV-2 Spike Antibody (3L24)
嗨！有任何问题？点我咨询
评论内容