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Anti-SARS-CoV-2 N Polyclonal Antibody

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货号 TMAB-01663

别名 Surface glycoprotein, SPIKE_SARS2, Spike protein S1, Spike protein RBD, Spike glycoprotein, SARS-CoV-2 spike protein, 2019-nCOV Spike protein

Anti-SARS-CoV-2 N Polyclonal Antibody 是一种 Rabbit 抗体，靶向 SARS-CoV-2 N。Anti-SARS-CoV-2 N Polyclonal Antibody 可用于 WB。

Anti-SARS-CoV-2 N Polyclonal Antibody

Anti-SARS-CoV-2 N Polyclonal Antibody

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货号 TMAB-01663 别名 Surface glycoprotein, SPIKE_SARS2, Spike protein S1, Spike protein RBD, Spike glycoprotein, SARS-CoV-2 spike protein, 2019-nCOV Spike protein

Anti-SARS-CoV-2 N Polyclonal Antibody 是一种 Rabbit 抗体，靶向 SARS-CoV-2 N。Anti-SARS-CoV-2 N Polyclonal Antibody 可用于 WB。

规格价格库存数量
100 μg
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-SARS-CoV-2 N Polyclonal Antibody is a Rabbit antibody targeting SARS-CoV-2 N. Anti-SARS-CoV-2 N Polyclonal Antibody can be used in WB.
别名Surface glycoprotein, SPIKE_SARS2, Spike protein S1, Spike protein RBD, Spike glycoprotein, SARS-CoV-2 spike protein, 2019-nCOV Spike protein
Ig Type
IgG
反应种属
(predicted:SARS-CoV-2)
验证活性
1. Sample:
Lane 1: SARS-CoV-2 N protein at 50ng
Lane 2: SARS-CoV-2 N protein at 25ng
Lane 3: SARS-CoV-2 N protein at 15ng
Lane 4: SARS-CoV-2 N protein at 10ng
Lane 5: SARS-CoV-2 N protein at 5ng
Lane 6: SARS-CoV-2 N protein at 2.5ng
Primary: Anti-SARS-CoV-2 N protein (TMAB-01663) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
2. Sample:
Lane 1: SARS-CoV N protein at 50ng
Lane 2: SARS-CoV N protein at 25ng
Lane 3: SARS-CoV N protein at 15ng
Lane 4: SARS-CoV N protein at 10ng
Lane 5: SARS-CoV N protein at 5ng
Lane 6: SARS-CoV N protein at 2.5ng
Lane 7: SARS-CoV-2 N protein at 50ng
Lane 8: SARS-CoV-2 N protein at 25ng
Lane 9: SARS-CoV-2 N protein at 15ng
Lane 10: SARS-CoV-2 N protein at 10ng
Lane 11: SARS-CoV-2 N protein at 5ng
Lane 12: SARS-CoV-2 N protein at 2.5ng
Primary: Anti-SARS-CoV-2 N protein (TMAB-01663) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
verifiedActivityverifiedActivity
应用WB
推荐剂量
WB: 1:500-2000
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion.
组织特异性The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protease t
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度≥1 mg/mL
研究背景The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.
抗原信息
免疫原
Recombinant Protein: SARS-CoV-2 Nucleocapsid Protein
基因名称
S
蛋白名称
Spike glycoprotein
研究领域
SARS Coronavirus
功能
attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.
化学信息
分子量Theoretical: 46 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

计算器

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