Anti-Sarcomeric Alpha Actinin Polyclonal Antibody is a Rabbit antibody targeting Sarcomeric Alpha Actinin. Anti-Sarcomeric Alpha Actinin Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Sarcomeric Alpha Actinin Polyclonal Antibody, Unconjugated (TMAB-01653) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: mouse embryos tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Sarcomeric Alpha Actinin Polyclonal Antibody, Unconjugated (TMAB-01653) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Sarcomeric Alpha Actinin) Polyclonal Antibody, Unconjugated (TMAB-01653) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Sarcomeric Alpha Actinin

Human

ACTN2

alpha-actinin-2

Cardiac arrhythmias,Actins,Actin Binding Proteins

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.