Anti-SAM68 Antibody (1C536) is a Rabbit antibody targeting SAM68. Anti-SAM68 Antibody (1C536) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.

IgG

1C536

Human,Mouse

1. Western blot analysis of A431 cell lysate. Using SAM68 (TMAB-12485) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (SAM68) monoclonal Antibody, unconjugated (TMAB-12485) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:1000-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human KHDRBS1

Human

KHDRBS1

KH domain-containing, RNA-binding, signal transduction-associated protein 1

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.
Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.