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Anti-S6K1/RPS6KB1 Polyclonal Antibody
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产品编号 TMAB-01647
Anti-S6K1/RPS6KB1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 S6K1/RPS6KB1。Anti-S6K1/RPS6KB1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-S6K1/RPS6KB1 Polyclonal Antibody
Anti-S6K1/RPS6KB1 Polyclonal Antibody
还可以产品编号 TMAB-01647
Anti-S6K1/RPS6KB1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 S6K1/RPS6KB1。Anti-S6K1/RPS6KB1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-S6K1/RPS6KB1 Polyclonal Antibody is a Rabbit antibody targeting S6K1/RPS6KB1. Anti-S6K1/RPS6KB1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,Goat) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RPS6KB1) Polyclonal Antibody, Unconjugated (TMAB-01647) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: MCF-7 (Human) Cell Lysate at 30 μg U-2Os (Human) Cell Lysate at 30 μg MOLT-4 (Human) Cell Lysate at 30 μg Primary: Anti-RPS6KB1 (TMAB-01647) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 68 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013] |
| 免疫原 | KLH conjugated synthetic peptide: human RPS6KB1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Rb,DNA Damage Recognition,Ribosome,Obesity,S6K Family |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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