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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-RPL5 Polyclonal Antibody

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货号 TMAB-12357

Anti-RPL5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 RPL5。Anti-RPL5 Polyclonal Antibody 可用于 IHC-P,WB,IHC-Fr,IF。

Anti-RPL5 Polyclonal Antibody

Anti-RPL5 Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-12357

Anti-RPL5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 RPL5。Anti-RPL5 Polyclonal Antibody 可用于 IHC-P,WB,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-RPL5 Polyclonal Antibody is a Rabbit antibody targeting RPL5. Anti-RPL5 Polyclonal Antibody can be used in IHC-P,WB,IHC-Fr,IF.
反应种属
Human,Mouse,Rat (predicted:Dog,Chicken,Cow,Horse,Rabbit,Sheep,Pig)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (RPL5) Polyclonal Antibody, Unconjugated (TMAB-12357) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (RPL5) Polyclonal Antibody, Unconjugated (TMAB-12357) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (RPL5) Polyclonal Antibody, Unconjugated (TMAB-12357) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (RPL5) Polyclonal Antibody, Unconjugated (TMAB-12357) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (RPL5) Polyclonal Antibody, Unconjugated (TMAB-12357) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Sample:
Lane 1: Human MOLT4 cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human U-2 OS cell lysates
Primary: Anti-RPL5 (TMAB-12357) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: kDa
Observed band size: 35 kDa
应用IHC-PWBIHC-FrIF
推荐剂量
IHC-P: 1:100-500; WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm. Nucleus
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
抗原信息
免疫原
KLH conjugated synthetic peptide: human RPL5
基因名称
RPL5
基因ID
6125
蛋白名称
60S ribosomal protein L5
功能
Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.
化学信息
分子量Theoretical: 34 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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