Anti-ROR alpha Polyclonal Antibody is a Rabbit antibody targeting ROR alpha. Anti-ROR alpha Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Heart tissue lysates
Lane 3: Human HeLa cell lysates
Primary: Anti-ROR alpha (TMAB-01629) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ROR alpha) Polyclonal Antibody, Unconjugated (TMAB-01629) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse ROR alpha

Mouse

RORA

Nuclear receptor ROR-alpha

Retanoic and retanoid,Cell differentiation,Cell Differentiation,Retinoic & Retinoid,Neural Signal Transduction

Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives.