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Anti-ROR alpha Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ROR alpha。Anti-ROR alpha Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-ROR alpha Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ROR alpha。Anti-ROR alpha Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,175 | 5日内发货 | |
100 μL | ¥ 1,960 | 5日内发货 |
产品描述 | Anti-ROR alpha Polyclonal Antibody is a Rabbit antibody targeting ROR alpha. Anti-ROR alpha Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Rat Heart tissue lysates Lane 3: Human HeLa cell lysates Primary: Anti-ROR alpha (TMAB-01629) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ROR alpha) Polyclonal Antibody, Unconjugated (TMAB-01629) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining. |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Nucleus. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
研究背景 | The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014] |
免疫原 | KLH conjugated synthetic peptide: mouse ROR alpha |
抗原种属 | Mouse |
研究领域 | Retanoic and retanoid,Cell differentiation,Cell Differentiation,Retinoic & Retinoid,Neural Signal Transduction |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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