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别名 S914, RGS 6, Regulator of G-protein signaling 6
Anti-RGS6 Antibody (5V916) 是一种抗体,靶向 RGS6。Anti-RGS6 Antibody (5V916) 可用于 ELISA,IF,IHC。
Anti-RGS6 Antibody (5V916) 是一种抗体,靶向 RGS6。Anti-RGS6 Antibody (5V916) 可用于 ELISA,IF,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
| 产品描述 | Anti-RGS6 Antibody (5V916) is an antibody targeting RGS6. Anti-RGS6 Antibody (5V916) can be used in ELISA,IF,IHC. |
| 别名 | S914, RGS 6, Regulator of G-protein signaling 6 |
| Ig Type | Rabbit IgG |
| 克隆号 | 5V916 |
| 交叉反应 | Human |
| 验证活性 | 1. IHC image of TMAH-01040 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. 2. IHC image of TMAH-01040 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. 3. Immunofluorescence staining of PC-3 cell with TMAH-01040 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 592-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| 应用 | ELISAIFIHC |
| 推荐剂量 | IHC:1:50-1:200; IF:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Cytoplasm, cytosol. Membrane; Peripheral membrane protein. Nucleus. Cell membrane. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS6/GNB5 dimer enhances GNAO1 GTPase activity. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human RGS6 |
| 抗原种属 | Human |
| 基因名称 | RGS6 |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Signal transduction |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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