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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-RASSF3 Polyclonal Antibody

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货号 TMAB-12120

Anti-RASSF3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 RASSF3。Anti-RASSF3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-RASSF3 Polyclonal Antibody

Anti-RASSF3 Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-12120

Anti-RASSF3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 RASSF3。Anti-RASSF3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-RASSF3 Polyclonal Antibody is a Rabbit antibody targeting RASSF3. Anti-RASSF3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
反应种属
Human,Mouse (predicted:Rat,Chicken,Dog,Cow,Horse,Rabbit)
验证活性
1. Sample: A549 Cell (Human) Lysate at 40 μg
Primary: Anti-RASSF3 (TMAB-12120) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
2. Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RASSF3) Polyclonal Antibody, Unconjugated (TMAB-12120) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample: Stomach (Mouse) Lysate at 40 μg
Primary: Anti-RASSF3 (TMAB-12120) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 30 kD
4. Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RASSF3) Polyclonal Antibody, Unconjugated (TMAB-12120) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RASSF3) Polyclonal Antibody, Unconjugated (TMAB-12120) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm. Cytoplasm, cytoskeleton. Note=Localized to microtubules in vascular endothelial cells.
组织特异性Widely expressed.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.[supplied by OMIM, Jul 2003].
抗原信息
免疫原
KLH conjugated synthetic peptide: human RASSF3
抗原种属
Human
基因名称
RASSF3
基因ID
283349
蛋白名称
Ras association domain-containing protein 3
Uniprot ID
Q86WH2
化学信息
分子量Theoretical: 26 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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