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Anti-QPRTase Polyclonal Antibody
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产品编号 TMAB-01599
Anti-QPRTase Polyclonal Antibody 是一种 Rabbit 抗体，靶向 QPRTase。Anti-QPRTase Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-QPRTase Polyclonal Antibody
Anti-QPRTase Polyclonal Antibody
还可以产品编号 TMAB-01599
Anti-QPRTase Polyclonal Antibody 是一种 Rabbit 抗体，靶向 QPRTase。Anti-QPRTase Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-QPRTase Polyclonal Antibody is a Rabbit antibody targeting QPRTase. Anti-QPRTase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Mouse,Rat (predicted:Human,Dog,Cow,Sheep) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Quinolinate phosphoribosyltransferase; QPRT/QAPRTase) Polyclonal Antibody, Unconjugated (TMAB-01599) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: Liver (mouse) Lysate at 40 μg Primary: Anti-QPRT/QAPRTase (TMAB-01599) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa 3. Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Large intestine tissue lysates Lane 3: Mouse Kidney tissue lysates Primary: Anti-QPRT/QAPRTase (TMAB-01599) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 28 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Hexamer formed by 3 homodimers. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 研究背景 | Quinolinate phosphoribosyltransferase (QPRTase) is a major enzyme in the catabolism of quinolinate. Quinolinate is an intermediate in the tryptophan-nicotinamide adenine dinucleotide (NAD) pathway, leading to the production of nicotinic acid, carbon dioxide, and pyrophosphate. Catabolism of quinolinate is vital due to the quinolinate’s neurotoxicity. Increased levels of quinolinate have been linked to neurodegenerative symptoms associated with meningitis and AIDS. QAPRTase has a seven-stranded alpha/beta-barrel domain which is similar in structure to the eight-stranded alpha/beta-barrel enzymes. The protein possesses a novel fold in comparison to other members of the PRTase family. This fold comprises a structure combining two domains. The structure is part alpha/beta barrel-like domain, and part alpha/beta N-terminal domain. |
| 免疫原 | KLH conjugated synthetic peptide: human QPRT/QAPRTase |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Amino acid metabolism,Neurodegenerative disease,Alzheimer's disease,Huntington's disease,Neurodegenerative disease,Amino Acids |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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