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Anti-PSME2 Antibody (7S935)
还可以产品编号 TMAY-02763
Anti-PSME2 Antibody (7S935) 是一种 Mouse 抗体，靶向 PSME2。Anti-PSME2 Antibody (7S935) 可用于 WB,ELISA,IP。
Anti-PSME2 Antibody (7S935)
Anti-PSME2 Antibody (7S935)
还可以产品编号 TMAY-02763
Anti-PSME2 Antibody (7S935) 是一种 Mouse 抗体，靶向 PSME2。Anti-PSME2 Antibody (7S935) 可用于 WB,ELISA,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-PSME2 Antibody (7S935) is a Mouse antibody targeting PSME2. Anti-PSME2 Antibody (7S935) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 7S935 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-PSME2 mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:27 kDa. -Observed band size:30 kDa. 2. PSME2 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-PSME2 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PSME2 mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 27 kDa. -Observed band size: 27 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PSME2 / PA28b (rh PSME2 / PA28b; TMPY-03610; Met1-Tyr239). The IgG fraction of the cell culture supernatant was purified by Protein A affinity. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | PSME2, also known as PA28b, is a subunit of proteasome. The 26S proteasome multicatalytic proteinase complex has a highly ordered structure composed of 2 complexes, a 2S core and a 19S regulator. The 2S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 1 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME2 gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human PSME2 / PA28b protein (TMPY-03610) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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