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Anti-PSGL-1/CD162 Antibody (9I582)
还可以产品编号 TMAY-02645
Anti-PSGL-1/CD162 Antibody (9I582) 是一种 Mouse 抗体，靶向 PSGL-1/CD162。Anti-PSGL-1/CD162 Antibody (9I582) 可用于 ELISA,ICC/IF。
Anti-PSGL-1/CD162 Antibody (9I582)
Anti-PSGL-1/CD162 Antibody (9I582)
还可以产品编号 TMAY-02645
Anti-PSGL-1/CD162 Antibody (9I582) 是一种 Mouse 抗体，靶向 PSGL-1/CD162。Anti-PSGL-1/CD162 Antibody (9I582) 可用于 ELISA,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-PSGL-1/CD162 Antibody (9I582) is a Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (9I582) can be used in ELISA,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 9I582 |
| 交叉反应 | Human |
| 验证活性 | Immunofluorescence staining of SELPLG in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human SELPLG monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
| 应用 | ELISA,ICC/IF |
| 推荐剂量 | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human PSGL-1/CD162 Protein (TMPY-03531) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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