Anti-PRPF8 Antibody (1C957) is a Rabbit antibody targeting PRPF8. Anti-PRPF8 Antibody (1C957) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

1C957

Human (predicted:Mouse,Rat)

1. 25 ug total protein per lane of various lysates (see on figure) probed with PRPF8 monoclonal antibody, unconjugated (TMAB-11822) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PRPF8 antibody (TMAB-11822, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human PRPF8

Human

PRPF8

Pre-mRNA-processing-splicing factor 8

Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.