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Anti-Protein A Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Protein A。Anti-Protein A Polyclonal Antibody 可用于 WB。
Anti-Protein A Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Protein A。Anti-Protein A Polyclonal Antibody 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,780 | 5日内发货 |
产品描述 | Anti-Protein A Polyclonal Antibody is a Rabbit antibody targeting Protein A. Anti-Protein A Polyclonal Antibody can be used in WB. |
Ig Type | IgG |
交叉反应 | S.aureus |
验证活性 | Sample: Lane 1: Recombinant protein A Primary: Anti-Protein A (TMAB-01581) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40-60 kDa Observed band size: 41 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Secreted; cell wall. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies. |
免疫原 | Recombinant Protein: protein A |
研究领域 | Protein A |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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