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Anti-Protein A Polyclonal Antibody

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Anti-Protein A Polyclonal Antibody
产品编号 TMAB-01581

Anti-Protein A Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Protein A。Anti-Protein A Polyclonal Antibody 可用于 WB。

Anti-Protein A Polyclonal Antibody

Anti-Protein A Polyclonal Antibody

Rating icon 还可以
Anti-Protein A Polyclonal Antibody
产品编号 TMAB-01581

Anti-Protein A Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Protein A。Anti-Protein A Polyclonal Antibody 可用于 WB。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-Protein A Polyclonal Antibody is a Rabbit antibody targeting Protein A. Anti-Protein A Polyclonal Antibody can be used in WB.
Ig Type
IgG
交叉反应
S.aureus
验证活性
Sample:
Lane 1: Recombinant protein A
Primary: Anti-Protein A (TMAB-01581) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40-60 kDa
Observed band size: 41 kDa
应用
推荐剂量
WB: 1:500-2000
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted; cell wall.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.
抗原信息
免疫原
Recombinant Protein: protein A
研究领域
Protein A
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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