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Anti-Prostate Specific Antigen Antibody (9D734)

货号 TMAB-11796 一键复制产品信息
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Anti-Prostate Specific Antigen Antibody (9D734) 是一种 Mouse 抗体，靶向 Prostate Specific Antigen。Anti-Prostate Specific Antigen Antibody (9D734) 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。

Anti-Prostate Specific Antigen Antibody (9D734)

一键复制产品信息
Rating icon 还可以
货号 TMAB-11796

Anti-Prostate Specific Antigen Antibody (9D734) 是一种 Mouse 抗体，靶向 Prostate Specific Antigen。Anti-Prostate Specific Antigen Antibody (9D734) 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。

Anti-Prostate Specific Antigen Antibody (9D734)
规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-Prostate Specific Antigen Antibody (9D734) is a Mouse antibody targeting Prostate Specific Antigen. Anti-Prostate Specific Antigen Antibody (9D734) can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Ig Type
IgG1
克隆号
9D734
反应种属
Human
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Prostate Specific Antigen) Monoclonal Antibody, Unconjugated (TMAB-11796) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Sample:
Lane 1: Prostate Specific Antigen (PSA) Protein at 500 ng
Primary: Mouse Anti-PSA Monoclonal Antibody at 1/1000 dilution (Cat. TMAB-11796)
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 32 kD
3. Measured by its binding ability in a indirect ELISA. Immobilized Human PSA (Native) at 2 μg/mL (100 μL/well) can bind Anti-Human PSA monoclonal antibody, the minimum detection concentration is 0.1 ng/mL
4. Sample:
Lane 1: Human Prostate Specific Antigen protein
Primary: Anti-Prostate Specific Antigen (TMAB-11796) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 35 kDa
5. Paraformaldehyde-fixed, paraffin embedded (Human Prostate Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Prostate Specific Antigen) Monoclonal Antibody, Unconjugated (TMAB-11796) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
应用WBIHC-PIHC-FrIFELISA
推荐剂量
WB: 1:500-2000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500; ELISA: 1:5000-10000
抗体种类
Monoclonal
宿主来源Mouse
亚细胞定位Secreted.
组织特异性Prostate.
构建方式Hybridoma Monoclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M PBS (pH7.4).
研究背景Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
抗原信息
免疫原
Prostate Specific Antigen (PSA) Antigen, Native
基因名称
KLK3
基因ID
354
蛋白名称
Prostate-specific antigen
功能
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
化学信息
分子量Theoretical: 23 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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