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Anti-PPARGC1A Polyclonal Antibody
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产品编号 TMAB-01570
Anti-PPARGC1A Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PPARGC1A。Anti-PPARGC1A Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
Anti-PPARGC1A Polyclonal Antibody
Anti-PPARGC1A Polyclonal Antibody
还可以产品编号 TMAB-01570
Anti-PPARGC1A Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PPARGC1A。Anti-PPARGC1A Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-PPARGC1A Polyclonal Antibody is a Rabbit antibody targeting PPARGC1A. Anti-PPARGC1A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| 交叉反应 | Human (predicted:Mouse,Rat,Pig,Goat) |
| 验证活性 | 1. Sample: Lane 1: Recombinant human PPARGC1A, N-Trx-His Primary: Anti-PPARGC1A (TMAB-01570) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kDa Observed band size: 61 kDa 2. A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PPARGC1A) polyclonal Antibody, Unconjugated (TMAB-01570) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:25-100; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene. |
| 免疫原 | KLH conjugated synthetic peptide: human PGC-1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Diabetes associated,Metabolism,Co-activators/co-repressors,Estrogen,Other factors,Energy Metabolism,Fatty acids,Mitochondrial markers,Fatty acid oxidation,Diabetes,Heart disease,Obesity,Energy Metabolism,Mitochondrial |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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