Anti-PP2A alpha/beta Antibody (1E786) is a Rabbit antibody targeting PP2A alpha/beta. Anti-PP2A alpha/beta Antibody (1E786) can be used in IF, IHC-Fr, IHC-P, IP, WB.

IgG

1E786

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with PP2A alpha/beta monoclonal antibody, unconjugated (TMAB-11647) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PP2A alpha/beta Monoclonal Antibody, Unconjugated (TMAB-11647) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PP2A alpha/beta Monoclonal Antibody, Unconjugated (TMAB-11647) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PP2A alpha/beta Monoclonal Antibody, Unconjugated (TMAB-11647) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PP2A alpha/beta Monoclonal Antibody, Unconjugated (TMAB-11647) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PP2A alpha/beta Monoclonal Antibody, Unconjugated (TMAB-11647) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PP2A alpha/beta Monoclonal Antibody, Unconjugated (TMAB-11647) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PP2A alpha/beta Monoclonal Antibody, Unconjugated (TMAB-11647) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human PP2A alpha

Human

PPP2CB

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform