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Anti-PNPLA2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 PNPLA2。Anti-PNPLA2 Polyclonal Antibody 可用于 WB,FCM。
Anti-PNPLA2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 PNPLA2。Anti-PNPLA2 Polyclonal Antibody 可用于 WB,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-PNPLA2 Polyclonal Antibody is a Rabbit antibody targeting PNPLA2. Anti-PNPLA2 Polyclonal Antibody can be used in WB,FCM. |
别名 | TTS2.2, Transport-secretion protein 2 (TTS2), PNPLA2, Pigment epithelium-derived factor receptor (PEDF-R), Patatin-like phospholipase domain-containing protein 2, Desnutrin, Calcium-independent phospholipase A2-zeta (iPLA2-zeta), ATGL, Adipose triglyceride lipase |
Ig Type | IgG |
交叉反应 | Human,Mouse (predicted:Rat) |
验证活性 | 1. Sample: Lane 1: Mouse Brown fat tissue lysates Lane 2: Mouse Heart tissue lysates Primary: Anti-Adipose Triglyceride Lipase (TMAB-01554) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 52 kDa 2. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Adipose Triglyceride Lipase antibody (TMAB-01554) Dilution: 3 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3 μg/test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
应用 | |
推荐剂量 | WB: 1:500-2000; FCM: 3ug/Test |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Lipid droplet. Cell membrane. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | Adipose triglyceride lipase (ATGL) may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity. ATGL catalyzes the initial step in triglyceride hydrolysis in mammalian adipose tissue. ATGL contains a patatin domain common to plant acyl hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals. |
免疫原 | KLH conjugated synthetic peptide: human ATGL |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Lipases,Lipases,Metabolic disorders,Obesity,Lipid metabolism |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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