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Anti-PMVK Antibody (7Y287) 是一种 Rabbit 抗体,靶向 PMVK。Anti-PMVK Antibody (7Y287) 可用于 WB,ELISA,IP。
Anti-PMVK Antibody (7Y287) 是一种 Rabbit 抗体,靶向 PMVK。Anti-PMVK Antibody (7Y287) 可用于 WB,ELISA,IP。
产品描述 | Anti-PMVK Antibody (7Y287) is a Rabbit antibody targeting PMVK. Anti-PMVK Antibody (7Y287) can be used in WB,ELISA,IP. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 7Y287 |
交叉反应 | Human |
验证活性 | 1. Anti-PMVK rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:21 kDa. -Observed band size:24 kDa(We are unsure as to the identity of these extra bands.) 2. PMVK was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg NIH-3T3 Whole Cell Lysate -0.5 µL anti-PMVK rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PMVK rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 21 kDa. -Observed band size: 21 kDa |
应用 | WB,ELISA,IP |
推荐剂量 | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.2-1 μL/mg of lysate |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human PMVK / phosphomevalonate kinase (rh PMVK / phosphomevalonate kinase; TMPY-04767; Q15126; Met1-Leu192). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis. |
偶联 | Unconjugated |
免疫原 | Recombinant Human PMVK / phosphomevalonate kinase Protein (TMPY-04767) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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