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Anti-PMVK Antibody (7Y287)

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产品编号 TMAY-02762

Anti-PMVK Antibody (7Y287) 是一种 Rabbit 抗体,靶向 PMVK。Anti-PMVK Antibody (7Y287) 可用于 WB,ELISA,IP。

Anti-PMVK Antibody (7Y287)

Anti-PMVK Antibody (7Y287)

Rating icon 还可以
产品编号 TMAY-02762

Anti-PMVK Antibody (7Y287) 是一种 Rabbit 抗体,靶向 PMVK。Anti-PMVK Antibody (7Y287) 可用于 WB,ELISA,IP。

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产品介绍

生物活性
产品描述
Anti-PMVK Antibody (7Y287) is a Rabbit antibody targeting PMVK. Anti-PMVK Antibody (7Y287) can be used in WB,ELISA,IP.
Ig Type
Monoclonal Rabbit IgG
克隆号
7Y287
交叉反应
Human
验证活性
1. Anti-PMVK rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:21 kDa.

-Observed band size:24 kDa(We are unsure as to the identity of these extra bands.)

2. PMVK was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-Lane B:0.5 mg NIH-3T3 Whole Cell Lysate

-0.5 µL anti-PMVK rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-PMVK rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 21 kDa.

-Observed band size: 21 kDa
应用
WB,ELISA,IP
推荐剂量
WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.2-1 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human PMVK / phosphomevalonate kinase (rh PMVK / phosphomevalonate kinase; TMPY-04767; Q15126; Met1-Leu192).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Human PMVK / phosphomevalonate kinase Protein (TMPY-04767)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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