Anti-PLVAP Polyclonal Antibody is a Rabbit antibody targeting PLVAP. Anti-PLVAP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Mouse,Rat (predicted:Human,Dog,Cow,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (TMAB-01552) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (TMAB-01552) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining.
3. Sample:
Lane 1: Mouse Prostate tissue lysates
Lane 2: Mouse Large intestine tissue lysates
Primary: Anti-PLVAP (TMAB-01552) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 62 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PLVAP

Human

Endothelial,Caveolae and Clathrin