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Anti-PLCZ1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 PLCZ1。Anti-PLCZ1 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。
Anti-PLCZ1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 PLCZ1。Anti-PLCZ1 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,970 | 5日内发货 | |
200 μL | ¥ 2,785 | 5日内发货 |
产品描述 | Anti-PLCZ1 Polyclonal Antibody is a Rabbit antibody targeting PLCZ1. Anti-PLCZ1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse) |
验证活性 | 1. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-PLCZ1 Polyclonal Antibody, Unconjugated (TMAB-01549) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PLCZ1) Polyclonal Antibody, Unconjugated (TMAB-01549) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining. |
应用 | |
推荐剂量 | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, PLCZ1 hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. It triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. PLCZ1 is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. It may exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. |
免疫原 | KLH conjugated synthetic peptide: human PLCZ1 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Metabolism of lipids and lipoproteins,Lipid metabolism |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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