Anti-PKM2 Antibody (5U320) is a Rabbit antibody targeting PKM2. Anti-PKM2 Antibody (5U320) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

5U320

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with PKM2 monoclonal antibody, unconjugated (TMAB-11500) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PKM2 Monoclonal Antibody, Unconjugated (TMAB-11500) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PKM2 Monoclonal Antibody, Unconjugated (TMAB-11500) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PKM2 Monoclonal Antibody, Unconjugated (TMAB-11500) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PKM2 Monoclonal Antibody, Unconjugated (TMAB-11500) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PKM2 antibody (TMAB-11500, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:50-200; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:1000-2000

Monoclonal

A synthesized peptide: human PKM

Human

PKM

Pyruvate kinase PKM

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.