Anti-PKA C alpha+beta Polyclonal Antibody is a Rabbit antibody targeting PKA C alpha+beta. Anti-PKA C alpha+beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow)

1. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PKA C alpha+beta) Polyclonal Antibody, Unconjugated (TMAB-11475) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: Brain (Rat) Lysate at 40 μg
Primary: Anti-PKA C alpha+beta (TMAB-11475) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40/50 kD
Observed band size: 50 kD
3. Sample:
Testis (Mouse) Lysate at 40 μg
Testis (Rat) Lysate at 40 μg
Primary: Anti-PKA C alpha+beta (TMAB-11475) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40/50 kD
Observed band size: 50 kD
4. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Rat Heart tissue lysates
Lane 5: Human MCF-7 cell lysates
Lane 6: Human Hela cell lysates
Lane 7: Human Huvec cell lysates
Primary: Anti-PKA C alpha+beta (TMAB-11475) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40/50 kD kDa
Observed band size: 50 kD kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PKA beta

Human

PRKACA

cAMP-dependent protein kinase catalytic subunit alpha

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis.