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Anti-PKA alpha/beta Polyclonal Antibody
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产品编号 TMAB-01543
Anti-PKA alpha/beta Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PKA alpha/beta。Anti-PKA alpha/beta Polyclonal Antibody 可用于 WB,ICC/IF。
Anti-PKA alpha/beta Polyclonal Antibody
Anti-PKA alpha/beta Polyclonal Antibody
还可以产品编号 TMAB-01543
Anti-PKA alpha/beta Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PKA alpha/beta。Anti-PKA alpha/beta Polyclonal Antibody 可用于 WB,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-PKA alpha/beta Polyclonal Antibody is a Rabbit antibody targeting PKA alpha/beta. Anti-PKA alpha/beta Polyclonal Antibody can be used in WB,ICC/IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Sample: Jurkat (Human) Cell Lysate at 40 μg Primary: Anti-PKA alpha + beta (TMAB-01543) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa 2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PKA alpha + beta) polyclonal Antibody, Unconjugated (TMAB-01543) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 3. Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Rat Liver tissue lysates Primary: Anti-PKA alpha + beta (TMAB-01543) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; ICC/IF: 1:100 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | oplasm. Cell membrane. Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity). |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKA beta (catalytic subunit) is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. |
| 免疫原 | KLH conjugated synthetic peptide: human PRKACA/PRKACB |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Integration of energy metabolism,Metabolism of lipids and lipoproteins,Serine/threonine kinases,Integration of energy,Lipid metabolism,Cancer,Kinases,PKA,PKC |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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