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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-PKA alpha/beta Polyclonal Antibody

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货号 TMAB-01543

别名 Protein kinase cAMP dependent catalytic beta, Protein kinase cAMP dependent catalytic alpha, Protein kinase A catalytic subunit beta, Protein kinase A catalytic subunit alpha, Protein kinase A catalytic subunit, PRKACB, PRKACA+PRKACB, PRKACA/PRKACB, PRKACA, PKACB, PKACA, PKA C-alpha, PKA C beta, PKA C alpha, PKA beta, PKA alpha+beta, PKA alpha, MGC9320, MGC48865, MGC41879, MGC102831, KAPCB, KAPCA, DKFZp781I2452, cAMP-dependent protein kinase catalytic subunit alpha, cAMP dependent protein kinase catalytic subunit beta, cAMP dependent protein kinase catalytic subunit alpha isoform 1, cAMP dependent protein kinase catalytic subunit alpha, cAMP dependent protein kinase beta catalytic subunit, cAMP dependent protein kinase alpha catalytic subunit

Anti-PKA alpha/beta Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PKA alpha/beta。Anti-PKA alpha/beta Polyclonal Antibody 可用于 ICC/IF,WB。

Anti-PKA alpha/beta Polyclonal Antibody

Anti-PKA alpha/beta Polyclonal Antibody

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货号 TMAB-01543 别名 Protein kinase cAMP dependent catalytic beta, Protein kinase cAMP dependent catalytic alpha, Protein kinase A catalytic subunit beta, Protein kinase A catalytic subunit alpha, Protein kinase A catalytic subunit, PRKACB, PRKACA+PRKACB, PRKACA/PRKACB, PRKACA, PKACB, PKACA, PKA C-alpha, PKA C beta, PKA C alpha, PKA beta, PKA alpha+beta, PKA alpha, MGC9320, MGC48865, MGC41879, MGC102831, KAPCB, KAPCA, DKFZp781I2452, cAMP-dependent protein kinase catalytic subunit alpha, cAMP dependent protein kinase catalytic subunit beta, cAMP dependent protein kinase catalytic subunit alpha isoform 1, cAMP dependent protein kinase catalytic subunit alpha, cAMP dependent protein kinase beta catalytic subunit, cAMP dependent protein kinase alpha catalytic subunit

Anti-PKA alpha/beta Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PKA alpha/beta。Anti-PKA alpha/beta Polyclonal Antibody 可用于 ICC/IF,WB。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,960
5日内发货
200 μL
¥ 2,785
5日内发货
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产品描述
Anti-PKA alpha/beta Polyclonal Antibody is a Rabbit antibody targeting PKA alpha/beta. Anti-PKA alpha/beta Polyclonal Antibody can be used in ICC/IF,WB.
别名Protein kinase cAMP dependent catalytic beta, Protein kinase cAMP dependent catalytic alpha, Protein kinase A catalytic subunit beta, Protein kinase A catalytic subunit alpha, Protein kinase A catalytic subunit, PRKACB, PRKACA+PRKACB, PRKACA/PRKACB, PRKACA, PKACB, PKACA, PKA C-alpha, PKA C beta, PKA C alpha, PKA beta, PKA alpha+beta, PKA alpha, MGC9320, MGC48865, MGC41879, MGC102831, KAPCB, KAPCA, DKFZp781I2452, cAMP-dependent protein kinase catalytic subunit alpha, cAMP dependent protein kinase catalytic subunit beta, cAMP dependent protein kinase catalytic subunit alpha isoform 1, cAMP dependent protein kinase catalytic subunit alpha, cAMP dependent protein kinase beta catalytic subunit, cAMP dependent protein kinase alpha catalytic subunit
Ig Type
IgG
反应种属
Human,Mouse,Rat
验证活性
1. Sample:
Jurkat (Human) Cell Lysate at 40 μg
Primary: Anti-PKA alpha + beta (TMAB-01543) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PKA alpha + beta) polyclonal Antibody, Unconjugated (TMAB-01543) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
3. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Primary: Anti-PKA alpha + beta (TMAB-01543) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
verifiedActivityverifiedActivityverifiedActivity
应用ICC/IFWB
推荐剂量
WB: 1:500-2000; ICC/IF: 1:100
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位oplasm. Cell membrane. Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity).
组织特异性Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKA beta (catalytic subunit) is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK.
抗原信息
免疫原
KLH conjugated synthetic peptide: human PRKACA/PRKACB
抗原种属
Human
基因名称
PRKACA
基因ID
5567
蛋白名称
cAMP-dependent protein kinase catalytic subunit alpha
Uniprot ID
P22694
研究领域
Integration of energy metabolism,Metabolism of lipids and lipoproteins,Serine/threonine kinases,Integration of energy,Lipid metabolism,Cancer,Kinases,PKA,PKC
功能
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis.
化学信息
分子量Theoretical: 40 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

计算器

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